Application

Anti-CD24 Antibody, clone SN3 detects level of CD24 & has been published & validated for use in FC, IP, IH.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Immunocytochemistry on frozen tissue sections.

Flow Cytometry: 1:50 - 1:100 per 10e6 cells in 100 µL final volume.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Glycoproteins purified from human NALM-1 cell line.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin by Protein G chromatography. Liquid in 1 mL of PBS, pH 7.4 containing no preservatives.

Format: Purified

Specificity

The antibody reacts with the CD24 antigen a heavily glycosylated molecule that migrates as a broad band of 35-45 kDa on both reducing and non-reducing SDS gel electrophoresis. CD24 is attached to the cell membrane via a glycosyl phosphatidylinositol (GPI) anchor and is expressed at multiple stages of B cell development, beginning with the bone marrow CD34 positive pro-B cell compartment and continuing through mature surface IgM positive/IgD positive B cells. CD24 is also expressed on the vast majority of B-lineage acute lymphoblastic leukaemias, B cell chronic lymphocytic leukaemias and B cell non-Hodgkin′s lymphoma. In the normal spleen, mantle zone lymphocytes are CD24 strongly positive, but germinal center cells are CD24 negative or CD24 weakly positive.

Storage and Stability

Maintain at -20°C for up to 12 months from date of receipt in undiluted aliquots.